Phos-tag SDS-PAGE database A-Raf

A-Raf

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Mw; 68,000

Image

HEK 293 cells were treated with Ser/Thr phosphatase inhibitor,calyclinA (100 nM) for 30 min, or Tyr phosphatase inhibitor, pervanadate (1 mM) for 30 mim, and then lyzed in SDS-PAGE loading buffer.

Reference	None
Composition of gel	Zn²⁺ -Phos-tag:　25µM Polyacrylamide: 8.5%(w/v)
Detection	WB: anti-A-Raf

Reference

None

Composition of gel

Zn²⁺ -Phos-tag:　25µM

Polyacrylamide: 8.5%(w/v)

Detection

WB: anti-A-Raf