Phos-tag SDS-PAGE database CHK1

CHK1

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA.
Mw; 54,400

Image

CHK1 is a substrate of ATR kinase, which responses DNA damage.

GFP-tagged chicken FANCI (GFP-chFANCI) was expressed in chicken DT40 cells ( disrupted endogeneous FANCI ), and the cells were treated MMC (DNA damaging agent).

Related data

　
FANCI

Reference	Ishiai, M., Kitao, H., Smogorzewska, A., Tomida, J., Kinomura, A., Uchida, E., Saberi, A., Kinoshita, E., Kinoshita-Kikuta, E., Koike, T., Tashiro, S., Elledge, S.J., and Takata, M. FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway. *Nature Structural & Molecular Biology*, 15, 1138–1146 (2008) DOI:10.1038/nsmb.1504
Composition of gel	Mn²⁺–Phos-tag:　3.5 µM Polyacrylamide: -
Detection	WB: anti-CHK1

Reference

Ishiai, M., Kitao, H., Smogorzewska, A., Tomida, J., Kinomura, A., Uchida, E., Saberi, A., Kinoshita, E., Kinoshita-Kikuta, E., Koike, T., Tashiro, S., Elledge, S.J., and Takata, M.
FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway.
Nature Structural & Molecular Biology, 15, 1138–1146 (2008)

DOI:10.1038/nsmb.1504

Composition of gel

Mn²⁺–Phos-tag:　3.5 µM

Polyacrylamide: -

Detection

　WB: anti-CHK1