Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mw; 130,000
HEK293 細胞を,Ser/Thr phosphatase 阻害剤のcalyculin A,またはTyr phosphatase 阻害剤のpervanadateで処理した後,JAK2のリン酸化状態の変化を Zn2+-Phos-tag SDS-PAGEで調べています。
Zn2+ -Phos-tag: 25µM
Polyacrylamide: 5%(w/v) + 0.5% agarose