Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Mw; 45,000
HEK293 細胞を,Ser/Thr phosphatase 阻害剤のcalyculin A,またはTyr phosphatase 阻害剤のpervanadateで処理した後,MAPKAPK2のリン酸化状態の変化を Zn2+-Phos-tag SDS-PAGEで調べています。
Zn2+ -Phos-tag: 25µM
Polyacrylamide: 8.5%(w/v)