Phos-tag SDS-PAGE database PKCζ

PKCζ

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP).
Mw; 68,000

Image

HEK293 細胞を，Ser/Thr　phosphatase 阻害剤のcalyculin A，またはTyr phosphatase 阻害剤のpervanadateで処理した後，PKC zetaのリン酸化状態の変化を
Zn²⁺-Phos-tag SDS-PAGEで調べています。

Reference	None
Composition of gel	Zn²⁺ -Phos-tag: 25µM Polyacrylamide: 6%(w/v)
Detection	WB: anti-PKC zeta

Reference

None

Composition of gel

Zn²⁺ -Phos-tag: 25µM

Polyacrylamide: 6%(w/v)

Detection

WB: anti-PKC zeta