Phos-tag SDS-PAGE database Raptor

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity
Mw; 149,000

HEK293 細胞を，Ser/Thr　phosphatase 阻害剤のcalyculin A，またはTyr phosphatase 阻害剤のpervanadateで処理した後，Raptorのリン酸化状態の変化を
Zn²⁺-Phos-tag SDS-PAGEで調べています。

Reference	None
Composition of gel	Zn²⁺ -Phos-tag: 25µM Polyacrylamide: 5%(w/v) + 0.5% agarose
Detection	WB: anti-raptor

Reference

None

Composition of gel

Zn²⁺ -Phos-tag: 25µM

Polyacrylamide: 5%(w/v)
+
0.5% agarose

Detection

WB: anti-raptor