Phos-tag SDS-PAGE database rictor (Erk2)

Phos-tag SDS-PAGE database rictor (Erk2)Phos-tag SDS-PAGE，phostag, phostag sds-page, Phos-tag, phosphorylated protein, phosphoprotein" />

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals.
Mw; 192,000

HEK293 細胞を，Ser/Thr　phosphatase 阻害剤のcalyculin A，またはTyr phosphatase 阻害剤のpervanadateで処理した後，rictorのリン酸化状態の変化を Zn²⁺-Phos-tag SDS-PAGEで調べています。

Reference	None
Composition of gel	Zn²⁺ -Phos-tag: 25µM Polyacrylamide: 5%(w/v) + 0.5% agarose
Detection	WB: anti-rictor

Reference

None

Composition of gel

Zn²⁺ -Phos-tag: 25µM

Polyacrylamide: 5%(w/v)
+
0.5% agarose

Detection

　WB: anti-rictor