Phos-tag SDS-PAGE database SRF

SRF

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
Mw; 52,000

Image

HEK293 細胞を，Ser/Thr　phosphatase 阻害剤のcalyculin A，またはTyr phosphatase 阻害剤のpervanadateで処理した後，SRFのリン酸化状態の変化を
Zn²⁺-Phos-tag SDS-PAGEで調べています。

Reference	None
Composition of gel	Zn²⁺ -Phos-tag: 25µM Polyacrylamide: 5%(w/v) + 0.5% agarose
Detection	WB: anti-SRF

Reference

None

Composition of gel

Zn²⁺ -Phos-tag: 25µM

Polyacrylamide: 5%(w/v)
+
0.5% agarose

Detection

WB: anti-SRF