Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors Mw; 87,000
HEK293 細胞を,Ser/Thr phosphatase 阻害剤のcalyculin A,またはTyr phosphatase 阻害剤のpervanadateで処理した後,STAT1のリン酸化状態の変化を Zn2+-Phos-tag SDS-PAGEで調べています。
Zn2+ -Phos-tag: 25µM
Polyacrylamide: 6%(w/v)