Phos-tag SDS-PAGE database A-Raf

Src

Proto-oncogene tyrosine-protein kinase Src.
Mw; 60,000

Image

FLAG-tagged Src was expressed inHEK 293 cells. Multiple phosphorylated forms were detected.

(G2A is non-myristoylated mutant)

Related data

Reference	Emiko Kinoshita-Kikuta, Toshihiko Utsumi, Aya Miyazaki, Chiharu Tokumoto, Kyosuke Doi, Eiji Kinoshita, Tohru Koike. Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during　intracellular protein traffic.Scientific Report (2021) https://doi.org/10.1038/s41598-020-73248-0
Composition of gel	Zn²⁺ -Phos-tag:　20µM Polyacrylamide: 7.5%(w/v)
Detection	WB: anti-FLAG

Reference

Emiko Kinoshita-Kikuta, Toshihiko Utsumi, Aya Miyazaki, Chiharu Tokumoto, Kyosuke Doi, Eiji Kinoshita, Tohru Koike. Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during　intracellular protein traffic.Scientific Report (2021)

https://doi.org/10.1038/s41598-020-73248-0

Composition of gel

Zn²⁺ -Phos-tag:　20µM

Polyacrylamide: 7.5%(w/v)

Detection

WB: anti-FLAG