Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Mw; 42,000
HEK293 細胞を,Ser/Thr phosphatase 阻害剤のcalyculin A,またはTyr phosphatase 阻害剤のpervanadateで処理した後,p42 MAPKのリン酸化状態の変化を Zn2+-Phos-tag SDS-PAGEで調べています。
Zn2+ -Phos-tag: 25µM
Polyacrylamide: 8.5%(w/v)