Phos-tag SDS-PAGE database Abltide GST inhibitor profiling

Abltide-GST

Recombinant fusion protein containing peptide EAIYAAPFAKKK with an N-terminal GST-tag, expressed in E. coli. Mr ~28,000

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Abltide_Glivec

Kinase inhibition assay of a tyrosine kinase, Abl, using
the substrate Abltide-GST and the specific inhibitor Glivec.

Reference	Kinoshita-Kikuta, E., Aoki, Y., Kinoshita, E., and Koike, T. Label-free kinase profiling usingphosphate affinity polyacrylamide gel electrophoresis. Molecular & Cellular Proteomics, 6, 356–366 (2007) DOI:10.1074/mcp.T600044-MCP200
Composition of gel	Mn²⁺–Phos-tag:　100µM Polyacrylamide: 12.5%(w/v)
detection	CBB stain

Reference

Kinoshita-Kikuta, E., Aoki, Y., Kinoshita, E., and Koike, T.
Label-free kinase profiling usingphosphate affinity polyacrylamide
gel electrophoresis.
Molecular & Cellular Proteomics, 6, 356–366 (2007)

DOI:10.1074/mcp.T600044-MCP200

Composition of gel

Mn²⁺–Phos-tag:　100µM

Polyacrylamide: 12.5%(w/v)

detection

CBB stain

Abltide-GST

Image

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