Phos-tag SDS-PAGE database Abltide GST kinase assay

Abltide-GST

Recombinant fusion protein containing peptide EAIYAAPFAKKK with an N-terminal GST-tag, expressed in E. coli. Mr ~28,000

Image

Phosphorylation of Abltide-GST incubated with tyrosine kinase Abl.

Mn²⁺-Phos-tag gel was analyzed by Westerm blotting with anti-pTyr antibody.

Reference	Kinoshita, E., Kinoshita-Kikuta, E., Takiyama, K., and Koike, T. Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Molecular & Cellular Proteomics, 5, 749-757 (2006) DOI:10.1074/mcp.T500024-MCP200
Composition of gel	Mn²⁺–Phos-tag:　100µM Polyacrylamide: 12.5%(w/v)
Detection	1.　CBB stain 2.　WB:　anti-pTyr antibody (clone PY20)

Reference

Kinoshita, E., Kinoshita-Kikuta, E., Takiyama, K., and Koike, T. Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Molecular & Cellular Proteomics, 5, 749-757 (2006)

DOI:10.1074/mcp.T500024-MCP200

Composition of gel

Mn²⁺–Phos-tag:　100µM

Polyacrylamide: 12.5%(w/v)

Detection

1.　CBB stain

2.　WB:　anti-pTyr antibody (clone PY20)

Abltide-GST

Image

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