Phos-tag SDS-PAGE database GSK3 beta

GSK3β

Protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules. Mw:47,000

Image

HeLa細胞を，Ser/Thr フォスファターゼ阻害剤のcalyculin A
あるいはTyrフォスファターゼ阻害剤のpervanadate で処理した場合の
細胞内GSK3β のリン酸化状態の変化をZn²⁺–Phos-tag SDS-PAGE調べています。

GSK3βについては，異なる実験条件で行ったデータも参照ください。

Related data

GSK3β

Reference	Kinoshita-Kikuta, E.,Kinoshita, E., Matsuda, A., Koike, T. Tips on Improvig the efficiency of electrotransfer of target proteins from Phos-tag SDS-PAGE gel. Proteomics, 14, 2437-2442 (2014) [supplemental data] DOI 10.1002/pmic.201400380 Kinoshita, E., Kinoshita-Kikuta, E., Koike, T. Improvig the electrotransfer efficiency of target phosphoprotein from Phos-tag SDS-PAGE gel. BUNSEKI KAGAKU, 64, 501-509 (2015) Phos-tag SDS-PAGE ゲルからの標的リン酸化タンパク質の転写効率改善分析化学,64,501-509（2015） https://doi.org/10.2116/bunsekikagaku.64.501
Composition of gel	Zn²⁺–Phos-tag:　25µM Polyacrylamide: 8%(w/v)
Detection	WB: anti-GSK3β

Reference

Kinoshita-Kikuta, E.,Kinoshita, E., Matsuda, A., Koike, T. Tips on Improvig the efficiency of electrotransfer of target proteins from Phos-tag SDS-PAGE gel. Proteomics, 14, 2437-2442 (2014)

[supplemental data]

DOI 10.1002/pmic.201400380

Kinoshita, E., Kinoshita-Kikuta, E., Koike, T. Improvig the electrotransfer efficiency of target phosphoprotein from Phos-tag SDS-PAGE gel. BUNSEKI KAGAKU, 64, 501-509 (2015)

Phos-tag SDS-PAGE ゲルからの標的リン酸化タンパク質の転写効率改善
分析化学,64,501-509（2015）

https://doi.org/10.2116/bunsekikagaku.64.501

Composition of gel

Zn²⁺–Phos-tag:　25µM

Polyacrylamide: 8%(w/v)

Detection

WB: anti-GSK3β