Phos-tag SDS-PAGE database A-Raf

Lyn

Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents.
Mw; 59,000

Image

FLAG-tagged Lyn was expressed inHEK 293 cells. Multiple phosphorylated forms were detected.

G2A is non-myristoylated mutant.

Arrowed bands are phospho-spesies phosphorylated myristolation dependent manner.

Related data

Reference	Emiko Kinoshita-Kikuta, Toshihiko Utsumi, Aya Miyazaki, Chiharu Tokumoto, Kyosuke Doi, Eiji Kinoshita, Tohru Koike. Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during　intracellular protein traffic.Scientific Report (2021) https://doi.org/10.1038/s41598-020-73248-0
Composition of gel	Zn²⁺ -Phos-tag:　20µM Polyacrylamide: 7.5%(w/v)
Detection	WB: anti-FLAG

Reference

Emiko Kinoshita-Kikuta, Toshihiko Utsumi, Aya Miyazaki, Chiharu Tokumoto, Kyosuke Doi, Eiji Kinoshita, Tohru Koike. Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during　intracellular protein traffic.Scientific Report (2021)

https://doi.org/10.1038/s41598-020-73248-0

Composition of gel

Zn²⁺ -Phos-tag:　20µM

Polyacrylamide: 7.5%(w/v)

Detection

WB: anti-FLAG